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When the sperm count is very low ,then a single sperm is injected in to the egg. Even if sperm are absent in ejaculate, then sperm can be extracted from testis ( Testicular Sperm extraction). it needs a micromanipulator with which an egg is held by suction and with a sharp needle a single sperm is injected and the fertilization is achieved. It is a boon for the couple where the routine IVF is not successful because of failed fertilization by conventional IVF or where the sperm count is very low or sperms are nonmotile.
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If
the female partner has both the fallopian tubes blocked or the
female is suffering from severe endometriosis or the husband is
very small number of motile and normal sperms or any other reason
by which the fertililization which occurs in outer part of the
fallopian tube does not take place.
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3.
MESA - Test tube baby
MESA
stands for microscopic epididymal sperm aspiration.
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It
is for those females who stopped menstruating either naturally or
prematurely and thus their stock of eggs are exhausted and they
wish to carry a pregnancy.
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This
method is meant for the cases where the female partner is not
releasing her eggs regularly leading to irregular periods with
long intervals.
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It
helps to store the sperms for long time and can be utilized
whenever required.
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To
see any cavity by a rigid or flexible telescope is known as
endoscopy, through a natural opening or making a small key hole.
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8. Blastocyst transfer
Blastocyst transfer is an unique method of transferring embryos
into the womb. In natural fertilization the uterus receives the
fertilized egg on 5/6th day after sperm fertilizes
the egg in woman’s fallopian tube at a stage called blastocyst.
In ICSI-IVF cycle we are now transferring blastocyst stage
embryos in to the womb by culturing them in sequential media for
5 days. It helps to select best growing embryos amongst the lot
and reduces the chances of multiple pregnancies. As the it is
more close to the natural fertilization , the implantation and
chances of getting a pregnancy is higher. For this long tem
culture we are now using triple gas incubators with reduced
oxygen concentration ( more close to natural environment )
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It
is a method by which the baby in the mother's womb can be
diagnosed whether it is suffering from thalessemia major or with
sickle cell disease. Tissue sampling is done by chronic villi
biopsy ( 8 - 10 weeks ) or by amniocentesis ( 11-14 weeks ).As it
represents the baby in-utero the analysis of the tissue helps to
diagnose the baby. The diagnosis is done by DNA PCR method. It is
most sophisticated method in the world and gives appropriate
results in 98-99 % cases.
Its
main activity is to diagnose anemia in females of reproductive
age group from macro to molecular (DNA ) level hence a proper
reason can be found out and the female can be treated
specifically.
In
cases of repeated abortions both the partners blood is checked
for any chromosomal defects by growing their blood cells
artificially .
In
cases of earlier birth of Down syndrome (kind of disease with
mental retardation )baby ,further birth of similar baby can be
prevented by analyzing the chromosomes of the earlier baby their
parents and if required of baby inside the uterus.
It
is to be incorporated very soon. It will give the diagnosis of
various genetic disorders in few hours (compared to days in
chromosomal analysis and DNA diagnosis )
The
center treats various hormonal diseased of the adolescents
regularly like increased hair growth at unwanted places
,irregular periods, obesity etc.
The
center is equipped with all kind of state of the art equipments
for DNA based pathology like for hepatitis, AIDS, malaria,
tuberculosis etc.
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