IVF & Genetic Center Jabalpur Madhya Pradesh, India

+  Intracytoplasmic Sperm Injection4
....................................
+  Test Tube Baby
....................................
+  MESA-IVF-ICSI
....................................
+  Ovarian Drilling
....................................
+  Egg-Donation
....................................
+  Embryo Freezing
....................................
+  Sperm Freezing
....................................
+  Endoscopy
....................................
+  Reconstructive
Surgery For Male
Infertility
....................................
+  Molecular Microbiology
....................................
+  Prenatal Diagnosis
....................................
+  Anemia Research
....................................
+  Chromosome Analysis
....................................

IDEAL FERTILITY CENTER - IVF & Genetic Center, Jabalpur 

SERVICES 44  FERTILITY CENTER
 

Intracytoplasmic Sperm Injection (ICSI)

When the sperm count is very low ,then a single sperm is injected in to the egg. Even if sperm are absent in ejaculate, then sperm can be extracted from testis ( Testicular Sperm extraction). it needs a micromanipulator with which an egg is held by suction and with a sharp needle a single sperm is injected and the fertilization is achieved. It is a boon for the couple where the routine IVF is not successful because of failed fertilization by conventional IVF or where the sperm count is very low or sperms are nonmotile.

ICSI

How ICSI is done in our Fertility Clinic ?

The stimulation protocol for the ICSI is same as that of conventional IVF. Eggs are surgically removed as in IVF . In normal circumstances the eggs are surrounded by cluster of cells called cumulus corona cells ,all of which must removed before the sperm injection takes place. Removal of the cells gives an insight of the eggs to embryologist ,to assess the maturity of the egg . Sperms are collected from the male partner , either by masturbation or they are surgically retrieved from testis.

Denuded eggs are placed in a dish under 200 or 400 magnification with a inverted microscope fitted with the micromanipulator .We have Nikon Narishige combinations of ultimate quality . The eggs is around 100 micron in width and is held by a glass holding pipette with a diameter of 40 to 50 micron usually on the left side . Injection needle is heving a diameter of five to six micron.

Sperms are first immobilized with a chemical (PVP ) and by hitting on the tail and loaded in the injection pipette. Injection is done with very fine movements of micromanipulator and usually it is completed with in a minute. It is the skill of the embryologist which matters the most. Fertilization is checked after 16-18 hours of injection.

Results: The fertilization rate of ICSI is around 65 % ( even though sperms are injected inside the eggs by us we should not expect 100 % fusion of both the gametes ).